›› 2009, Vol. 40 ›› Issue (6): 886-890.doi: 10.3969/j.issn.0529-1356.2009.06.008

• 论著 • 上一篇    下一篇

大鼠脑缺血再灌注后诱导型环氧合酶和丙二醛的表达及川芎嗪的干预作用

杨迎春1;任占川1*; 梁勇刚2   

  1. 1.山西医科大学汾阳学院解剖学教研室;2.形态学实验室,山西 汾阳 032200
  • 收稿日期:2008-09-14 修回日期:2009-11-24 出版日期:2009-12-06
  • 通讯作者: 任占川

Study of the expression of cyclooxygenase-2, malonic dialdehyde and the protective effect of Tetramethylpyrazine after cerebral ischemic-reperfusion in rat

  1. Department of Anatomy, Fenyang School of Medicine, Shanxi Medical University, Shanxi Fenyang 032200, China
  • Received:2008-09-14 Revised:2009-11-24 Online:2009-12-06
  • Contact: REN Zhan-chuan

关键词: 脑缺血, 诱导型环氧合酶, 丙二醛, 川芎嗪, 免疫印迹法, 硫代巴比妥酸法, 大鼠

Abstract: Objective To investigate the expression, relationship, significance of cyclooxygenase-2 (COX-2) and malonic dialdehyde (MDA) after cerebral ischemic-reperfusion injury and provide basis of treatment. Methods The focal cerebral ischemic-reperfusion model was established with thread embolish of middle cerebral artery. Western blotting, barhituric acid method and neurological evaluation were used to examine the expression of COX-2, MDA in cortex and the changes of neurological function; TTC staining was used to observe the changes of cerebral infarction volume. Results COX-2 prorein expression was correclated well with the MDA(EM>r/EM>=0.910,EM>P/EM>0.01). The content of COX-2 and MDA was very low in sham operation group, they were increased significantly at I2h/R6h model group, with the increase of reperfusion time,they remarkably reached its peak at I2h/R24h,they were slightly lower at I2h/R48h, but still maintained at a high level;Compared with model group, in tetramethylpyrazine(TMP) treatment group, the content of MDA and cerebral infarction volume were markedly decreased(EM>P/EM>0.01). Conclusion The expression of COX-2 and MDA increases in cerebral ischemic-reperfusion injury.It indicates they may play an important role in the mechani

Key words: Cerebral ischemic, Cyclooxygenase-2, Malonic dialdehyde, Tetramethylpyrazine, Western blotting, Barhituric acid method, Rat

中图分类号: